NEU259: Introduction to Light and Electron Microscopy (Spring 2011)
Instructors:
Gina Sosinsky, Ph. D.; gsosinsky@ucsd.edu, X40128
Mark Ellisman, Ph. D.; mellisman@ucsd.edu, X41392
Time and Location: Tuesday/Thursday 10:00 - 11:00 am, 1000 BSB Conference Room
Thursday demo: 11:00 - 12:30, Location to be announced
Syllabus: (document download)
Readings: Readings will be assigned by instructor and will be available with our administrative assistants for the NCMIR facility. A web course on electron microscopy can be found at http://em-outreach.ucsd.edu.
Our course websites can be accessed at: http://ncmir.ucsd.edu/training/ucsd_courses/index.shtm
No. |
Date |
Topic |
Lecturer |
Demo |
1 |
29-Mar |
Intro to Course / Intro to Optics I |
Gina Sosinsky/ James Bouwer |
|
2 |
31-Mar |
Intro to Optics II |
James Bouwer |
NCMIR Tour - ME |
3 |
5-Apr |
Confocal Microscopy /Fluorescence |
Tom Deerinck |
|
4 |
7-Apr |
Transmission Electron Microscopy |
Tom Deerinck |
LM - HH/TD |
5 |
12-Apr |
Multiphoton Miroscopy |
James Bouwer |
|
6 |
14-Apr |
Optical probes for live imaging |
Varda Lev-Ram |
TEM - TD |
7 |
19-Apr |
Specimen Preparation |
Gina Sosinsky |
|
8 |
21-Apr |
Immunocytochemistry for LM and EM |
Gina Sosinsky |
Confocal – Hiro Hakozaki |
9 |
26-Apr |
In Situ Hybridization *** CLASS in MTF168 *** |
Krista Todd |
|
10 |
28-Apr |
Midterm |
|
No Demo |
11 |
3-May |
Advance Light Microscopy Techniques (e.g. TIRF, near field) |
Hiro Hakozaki |
|
12 |
5-May |
Digital Imaging |
James Bouwer |
AFM demo |
13 |
10-May |
Image Processing/Analysis Fundamentals |
Gina Sosinksy |
|
14 |
12-May |
Deconvolution Microscopy |
James Bouwer |
Adobe Photoschop/Deconvolution Demo ImageJ (Hakozaki) |
15 |
17--May |
Scanning Probe Microscopies |
Gina Sosinsky |
|
16 |
19-May |
Electron Tomography |
Guy Perkins |
Tomography |
17 |
24-May |
Molecular Microscopy: Specimen Prep |
Gina Sosinsky |
|
18 |
26-May |
Molecular Microscopy: 3D Recon Methods |
Tim Baker |
SEM demo - TD |
*19 |
31-May |
Meso-scale imaging techniques & Future of Microscopy |
Mark Ellisman |
|
20 |
2-Jun |
OPEN |
|
|
** Finals Week |
9-Jun |
Student Presentations |
All instructors |
|
* Written assignment "My thesis project and how microscopy can contribute to my thesis." (1 page limit is for the body of writing. Use Arial font, 11 pt, 1 inch margins (2.5 cm) relative to 8.5 x 11 inch paper format. Line spacing should be 6 lines/inch (use single spacing with Microsoft Word).
**Final examination is take-home and due back by June 11th.
